A Walk in Tbilisi with Gagosh Georgias Political Stencil King

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“Do you consider this street poetry? It is my job to seize their attention, be a street terrorist. “This is the underlying idea behind street art. We come to a bridge and cross to the left bank of the Kura River, but under the yellow streetlamps, I see writing on the stone railing and sidewalk. “City Hall commissions commercialized street art and erases our graffiti in the tunnels.” Here the Russian doll says, ‘we are friends, we bring money, we are nice people.’ But inside her is all the war.

microRNAs play critical roles in the survival and recovery of Caenorhabditis elegans from starvation-induced L1 diapause

Compromising overall miRNA function dramatically reduces the survival rate of L1 worms in starvation-induced diapause, and the effect can be significantly suppressed by an age-1/PI3K mutation. Furthermore, miR-71 plays a prominent role in developmental recovery from L1 diapause partly through repressing the expression of certain heterochronic genes. When you restore a backup that contains third-party account information you must enter the recovery password to decrypt the backup. If you opt-in to third-party account backup and restore, and have set an account recovery password, then the app backups to Google Drive (Android) or iCloud (iOS) do include the private key information for your third-party accounts.
We further examined worms recovering from 4 d of L1 starvation and found that around 90% of the mir-71(lf) mutants displayed retarded vulval precursor cell (VPC) division, compared with less than 5% in wild type (Fig. 4A). We found that the 3′UTRs of several genes of the InsR pathway, including unc-31, age-1, pdk-1, akt-2, and sgk-1, contain predicted miR-71 targeting sites (as predicted by TargetScan and mirWIP). (H and I) Fluorescence images (H) and statistical data (I) showing that the M cell diveded in fed animals but remained undivided in 4-, 7-, or 11-d–starved L1 wild-type and mir-71(lf) worms. (E) Fluorescence and DIC images showing that the unc-31 3′UTR reporter was repressed in mir-71(+)worms (2/2 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines). We found that the poor survival rate of daf-16(mu86)(lf) was further decreased by mir-71(lf) (Fig. 2C), consistent with the notion that a portion of miR-71 activities regulate genes that act in parallel to UNC-31–mediated InsR/PI3K signaling for long-term survival during L1 diapause. Mutating miR-71 drastically reduces the survival rate of animals in L1 diapause, and the effect can be suppressed by mutations of insulin receptor pathway genes age-1 and unc-31.
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• The eggs were transferred to plates seeded with HB101 and bleached again 3 d later.
• We identified 10 miRNA mutants that showed reduced survival rates with a stringent standard, as well as a few miRNA mutants with slightly increased survival rates (Table S1, Fig. 1D, and Fig. S1B).
• (C) Fluorescence and differential interference contrast (DIC) images showing that the age-1 3′UTR reporter was repressed in mir-71(+) worms (3/4 transgenic lines) but not in mir-71(lf) worms (4/4 transgenic lines).
• Elegans Genetic Center (reference 257) and an N2 strain from the laboratory stock, respectively.
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Furthermore, worms that are long-lived due to dietary restriction or decreased mitochondrial respiratory rates are short-lived during L1 diapause, suggesting that the mechanisms controlling L1 starvation survival are different at least in some aspects from those controlling aging (3). We provide evidence that miRNA miR-71 is not required for the animals’ entry into L1 diapause, but plays a critical role in long-term survival by repressing the expression of insulin receptor/PI3K pathway genes and genes acting downstream or in parallel to the pathway. Full device encrypted backups to iTunes will back up both the account listings and private key pairs, but can only be restored on the SAME phone that created the backup. If you enabled third-party account backup, you can recover your accounts on your iOS or Android device. If your organization hasn’t enabled self-service device management, contact your IT Help Desk or Duo service administrator for assistance reactivating the account.

miR-71 Is Not Required for Arresting Seam Cell or M-Cell Divisions During L1 Diapause.

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miR-71 Likely Directly Represses the Expression of age-1 and unc-31 by Acting on Their 3′ Untranslated Regions.

A recent study showed that the expression of miR-71 was significantly increased relative to other miRNAs in starved L1 worms (15). However, miR-71 does not appear to regulate all postembryonic development during L1 diapause recovery. Unlike classical heterochronic miRNAs such as lin-4 and let-7, the role of miR-71 in vulval cell division is essential in animals recovering from starvation-induced L1 diapause, but not in animals hatched on plates with food. As pointed out above, multiple miRNAs in addition to miR-71 and the let-7 family miRNAs have roles in L1 diapause, and they may regulate the expression of many diverse targets that may include, but are not limited to, factors involved in UNC-31–InsR-signaling activities.

Using multiple methods

On the other hand, the role of a particular miRNA (miR-71) is executed by repressing the expression of many genes in multiple pathways. On one hand, we showed that deletions of a good number of miRNAs have varying impacts on the L1 diapause survival rate, although they may effect the rate through different mechanisms. Instead, many specific physiological functions, such as the starvation-induced stress response, are regulated by a miRNA-target network, often involving multiple miRNAs and a large number of their targets. We found that the known developmental timing genes, hbl-1, lin-42, and lit-1, were at the top of the list (TargetScan). To understand how miR-71 affects VPC division, we searched its predicted targets for potential genes involved in regulating developmental timing. These results indicate that miR-71 plays a significant role in larval development of animals recovering from L1 diapause and likely does so by regulating the expression of components of the insulin receptor/DAF-16 pathway, as well as factors acting downstream, or in parallel to, DAF-16.
The reporter construct, the control plasmid, and a transformation marker plasmid were coinjected into worms to generate the extrachromosomal arrays for analysis. Elegans and Caenorhabditis briggsae, leading us to focus further analyses on these two genes. We further examined the functional relationship between miR-71 and DAF-16, a FOXO transcription factor acting critically and negatively downstream of AGE-1/PI3K in the InsR pathway. Because the InsR pathway was previously shown to play a prominent role in L1 diapause (2, 3), we examined genetic interactions between miR-71 and different components of the InsR pathway.

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With both her artwork and writing, Helene seeks to provoke thought and inspire dialogue. Helene is a writer and artist based in New York City. From childhood drawings in a classroom mock economy to murals painted across the country, from sketchbooks filled with shadows to students learning to make their own, Josh McQuary has made art both his anchor and his offering. Whether he’s exploring the quiet magic of childhood fantasy, confronting political reality, or inviting strangers to tell their own stories, Josh treats art not just as a form of expression but as a conversation. The title refers to the realization that every stranger lives a life as vivid and complex as your own. Over time, his work became more political, blending symbolism with social commentary.

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They exhibited his work at Art Wynwood, Miami and soon at Spectrum art fair Miami. He is also part of Colour Senses Project since February 2024. The artist is represented by Miart Gallery London.
“I just picked up some watercolors and inks and started making literally darker paintings.” The response was overwhelming, and it sparked a major shift in his style. Josh’s career reached a major turning point in 2017 when he won the Pabst Blue Ribbon Art Contest, which put his artwork on over a million cans nationwide. Once there, he found not only fellow artists but also mentors within the local street art scene who took him under their wing. “They were like, ‘Dude, this is a really artsy city. After his service, Josh moved frequently, living in places like Colorado, California, and Chicago, searching for creative communities that could support his vision. At 19, McQuary enlisted in the military and was deployed to Afghanistan, an experience that fundamentally reshaped his worldview and relationship to creativity.

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While other students opened bakeries and clothing stores, McQuary and a friend launched an art commission booth. He’s been making art for as long as he can remember, but one pivotal moment came during a mock economy project in elementary school. Josh’s evolving style, practice, and motivation are inseparable from his life experiences and a growing desire to share not just the finished product, but the process and purpose behind it. While his art may seem rooted in metaphor and fantasy, it’s deeply personal. Off the street, he’s known as Josh McQuary, a Portland-based artist whose distinctive black-and-white inkwork channels the weight of fables, dreams, and political upheaval.

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